ISAS V1

Multispecies sperm analysis: ISAS V1

ISAS V1 for Sperm tests, which are increasingly widespread evidence among the animal and human population. Hence, there is an increasing supply of systems on the market and, by extension, it is increasingly necessary to have systems that offer greater efficiency in the results obtained.
In Proiser we are aware of this, that is why we have developed the ISAS V1 method, a system that gathers tools to be used both in clinics and in research.

Its fields of application are both the field of human sciences and those related to veterinary, scientific research, livestock production or pharmaceutical and reproductive toxicology.

ISAS V1 is currently considered to be the most complete and easy-to-use sperm analysis system of all we can find on the market. This is mainly because it works under different hardware conditions and with different operating systems, so it adapts to the needs of all types of users.

Caption from DNA Fragmentation-. ISAS V1 

ISAS V1: What it is and what applications it has

ISAS V1 is a sperm analysis system suitable for multiple species, so it can work with different sperm samples. It doesn’t matter if it is to analyze the sperm sample of a man, a horse, a bull, a pig, a goat, rabbit, dog, rat, lamb, etc. It’s valid for all of them. This does not imply that ISAS V1 always applies the same parameters. On the contrary, each species has its own configuration algorithms and results in independent analysis.

Depending on the sample, ISAS may use negative or positive phase contrast. The motility analysis of a field is performed in real time (less than one second), and can be configured to analyze motility up to 250 frames, at 200 frames per second and with concentrations greater than 1000 sperm/field.

In addition to the concentration, motility and percentage of progressives, with ISAS V1 the following kinetic parameters are also calculated: VCL, VSL, VAP, LIN, STR, WOD, ALH, BCF, DNC, MAD, among others.

It should also be noted that it works with cameras up to 200 frames per second, allowing for near-perfect analysis of sperm behavior.

In addition, it is specially designed for quality control programs, allowing the analysis of video files of many different formats (Windows AVI, Windows Media Video, MPEG-4, DV among others). It also allows to obtain images, which makes it easier to have collections of videos and images for offline analysis of mobility, concentration, morphology, vitality or DNA fragmentation.

Another application of ISAS V1 is the morphological and morphometric analysis of sperm from dyed or fresh samples (using phase contrast optics).
This work provides data on 14 morphometric parameters: head size (area, length, width and perimeter), head shape (ellipticality, elongation, roughness, and regularity), percentage of acrosome, head color level, centerpiece size (area and width), and insertion of the middle part (angle and distance).

With ISAS we can also know the data related to the percentage of fragmented sperm. To do this, it uses 20x or 40x real-time (less than 1 second) targets.

Finally, it should be noted that ISAS V1 also provides data on automatic feasibility of sperm cells. This is especially interesting because we need to determine the actual percentage of viable cells in a sample, and to accurately perform this analysis, we use fluorescence techniques with their corresponding use of tying kits.

ISAS V1

Caption From Vitality test-. ISAS V1

FEATURES of the ISAS V1 system

The current ISAS V1 equipment is completely modular, so each part of it can operate independently and together.
In addition, we have designed the system to allow most of the components that can be found in each type of laboratory, from the microscope to the computer, as long as they meet the minimum system requirements.

So, if you are looking for a system with which you can do high quality multispecies sperm analysis and be able to obtain a large number of data, do not hesitate and come to Proiser. You’ll find everything you need.

 

Isaspsus analisis s

Isaspsus, to analyse Swine Semen

Artificial animal insemination currently has effective methods that multiply the chances of new members developing. One of them is the Isaspsus method, with the analysis of the semen of pigs is not only a simple act but allows to obtain a lot of data about their quality and possibilities of reproduction.

isaspsus

CASA SYSTEM ( Sperm Analyzers),  Isaspsus Method for swine semen

 Isaspsus Method

 Isaspsus  Method to analyze swine semen,  is a quiality meassuring system with which you want to know its composition and calibrate its quality before being used in the insemination processes of females. 

This system is based on a new concept with which sperm selection is carried out. A methodology based on simultaneous analysis of mobility and cell-by-cell morphology. And all of this analyzing thousands of sperm per second.

The parameters that allow to identify and know the Isaspsus method are the following:

  • The total number of sperm (per milliliter and per ejaculate).
  • The progressive movement of all of them (per milliliter and by ejaculate).
  • The morphology of the sperm tail (per milliliter and by ejaculate).
  • Total useful sperm (per milliliter, by ejaculate and per dose).
  • Tail abnormalities.
  • Cytoplasmic drops.

 

 

This method allows seminal dose production to be significantly increased by up to 40%. However, it is not the only advantage as it presents many others such as the following:

  • Calculate precise doses that we need in each case.
  • Develop real-time, non-dying morphometry detection.
  • Be able to recognize cytoplasmic drops.
  • Develop tail analysis
  • Definition of the useful sperm in each sample.
  • We can save images and results to the database we handle.
  • We can repeat the function every day, every month, or annually; and even do it on samples of the same animal
  • We can get reports in Excel to visualize the data more clearly.

 

isaspsus-CASA

 CASA System-. Sperm Analyzers

Components

The Isaspsus method consists of the following elements to perform its function:

  • A Proiser M13-ON camera. This is a color camera, with a 1/2″ image sensor; resolution of 1280-1024, or what is the same as 8/10 bits per pixel; allowing you to offer frames up to 120 frames per second; Which consumes 1.3 watts and features a C lens mount.
  • An UB 203i microscope. It is a tri-ocular model, with voltage protector to homogenize the fluctuations of light and prolong the life of the LED; with a heating plate system; negative phase contrast; quintuple revolver; and reflected fluorescence accessory for the vitality or motility module; concentration using Hoechst.
  • A 22-inch Lenovo monitor, 4 Gigs capacity, a 500-mega hard drive, running Windows 10 Pro 64-bit.

A winning team with which you will not miss a detail of your animal semen samples, being able to shuffle a greater number of data that participate in the reproductive process. Which greatly multiplies your chances of success.

In addition, the cell of each sample is amplified by hovering the mouse over. And colors and shapes make the difference from the sperm found under the microscope.

If you are interested in purchasing the Isaspsus method, do not hesitate and contact us In Proiser we have everything you need and much more. Remember that we are a company that generates innovative solutions that lead to great improvements in sperm analysis procedures. This saves significant time in obtaining data on sperm mobility, sperm motility, sperm morphology or sperm concentration.

 

So if you want to know more about this method or many of our proposals, you only have to visit us. You won’t regret it!

 

spermtrack counting chamber

Combined effects of type and depth of counting chamber, and rate of image frame capture, on bull sperm motility and kinematics. Semen quality assessment requires accurate, reliable and objective methods.

Semen quality assessment requires accurate, reliable and objective methods for examination of sperm variables including sperm motility. For preparation of semen samples for artificial insemination, as a genetic resource, samples that are used for insemination need to have the capacity to result in a highly acceptable fertility rate. Several methods have been developed for evaluation of bull sperm in laboratory conditions and for preparation of doses for artificial insemination.

                       

spermtrack counting chamber

Spermtrack®

Computer-assisted semen analyses can provide objective information on various sperm variables. Nevertheless, this equipment requires fine-calibrations considering differences among species, breeds and conditions for sample evaluation and data analyses. In the present study, there was examination of the interaction between factors such as image frame rate and type and depth of counting chamber in which sperm were evaluated, together with differences between bulls of four breeds. The use of the Spermtrack® reusable 10 μm-depth  counting chamber provided more reliable results than results obtained using disposable counting chamber (10 and 20 μm depth). A capture rate of at least 90 fps is required for assessment of sperm motility percentage, whereas a rate of 250 fps is needed for obtaining consistent kinematic data. Differences among breeds in the present study indicate conditions for sperm analyses should include specific equipment calibrations for each breed. These results contribute to development of more precise conditions for assessments of bull sperm quality taking into account breed differences and the requirement each breed has for the adequate evaluation and preparation of samples for artificial insemination.

computer assisted counting tra

Computer Aided Sperm Analysis (CASA)

  

 

combined effects of counting chamber

Dowload PDF file for more information.

Influence of the microbiota of the reproductive tracts of females and males of the sheep species on their fertility

Using the technique of massive sequencing, interesting results over the influence of the microbiota of the reproductive tracts of females and males of the sheep species on their fertility.

Artificial insemination (AI) is an essential tool in genetic improvement programs, especially in dairy ruminants. In sheep, AI fertility has low percentages ranging between 30% and 60%, there are many factors that contribute to this fact.

The majority of fertility-related characters show very low heritability, between 1 and 4% (Andersen-Ranberg et al. 20015; Chang et al. 2006; González-Recio et al. 2006), which, together with The difficulty and time required for the collection of phenotypes, and the complexity and impression of the statistical models for their evaluation, make the selection of these characters using traditional methods inefficient.

Traditionally, the complete microbiota of the tract is not cultivable, so it has been tried to find out by other methodologies.

The INIA Animal genetic improvement group have found good conclusions about the influence of the microbiota of the reproductive tracts of females and males of the sheep species on their fertility , but there is still much to work on to clarify these issues and improve sheep production as in other species.

Information: Publication for veterinarians of Production animals, albéitar (November 2019, vol. 230)

Related posts: https://proiser.com/determine-the-semen-quality-of-three-species/

Influence of the microbiota

How do you do stain to evaluate sperm morphology?

For the correct visualization of a sperm sample it is important to know how to stain to evaluate sperm morphology. Approximately 15% of couples have infertility problems. Morphology is an important parameter for successful fertilization.

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ISAS V1

Sperm analysis CASA system: ISAS V1

A sperm analysis CASA system is one whose fundamental objective is to perform an accurate analysis, based on the repetition and performed automatically, of some parameters that appear in a sperm sample.

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Determine the semen quality of three species of interest to science with ISAS® V1

European scientists create a system for determining the quality of individualized semen. The study, the quality of sperm samples from three species of great interest was determined for conservation reasons or for the interest of the industrial aquaculture sector.

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Combined effect of counting chamber and diluent on sperm kinematics

The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination.

The evaluation of sperm The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination. Here, the combined effect of the type and capture area of three counting chambers, together with the type of diluent employed, on sperm motility was analysed. Ejaculates from thirteen Holstein bulls were used for sperm kinematic analysis with the ISAS®v1 CASA‐Mot system, using two capillary‐loaded counting chambers (Leja® and Cell‐Vu®) and one drop displacement chamber (Makler®).

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ISASP – The most successful solution for evaluation of seminal samples

A system for evaluation of seminal samples that will help you save a lot of time in the analysis of your samples for the doses that you need.

Proiser provides you with a series of solutions for the evaluation of seminal samples of any kind. As we have previously showed, our ISASv1 software is multi-species and gives you the possibility to acquire a lot of information about the sample.

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Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence

Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence (12-18 years) and the effect of varicocele.

Volunteers between 150 and 224 months of age, who had reached oigarche by 12 years old, were recruited in the area of Barranquilla, Colombia.

Genetic, environmental, and nutritional factors have a great influence on the time to reach puberty. Therefore, it is difficult to predict the age at which a particular individual will achieve his reproductive maturity, i.e., the sperm production corresponding to that of an adult.

In addition, biomedical information about the age of first conscious ejaculation (oigarche1), the presence or absence of spermatozoa in seminal fluid, the biological characteristics of spermatozoa during this maturational process, and the time required to reach adult spermatogenesis, is currently scarce and controversial.

The few studies reported on this topic correspond to adolescents under the age of 18 years and are related to different pathologies such as testicular cancer.

The main objective of this study was to investigate sperm features in adolescents between 12 and 18 years of age. These data are part of a social project carried out in Barranquilla, Colombia, directed to develop a sexual educational program because of the high teenage pregnancy rate in this area. Therefore, it is necessary to know the mean age at the onset of sperm production in adolescents from this area to provide the best counseling.

In addition, we analyzed the effect of varicocele and used morphometric data analysis to assess sperm subpopulations in human semen samples from these adolescents.

Complete article: https://proiser.com/wp-content/uploads/2019/06/Articulo-adolescentes.pdf