Influence of the microbiota of the reproductive tracts of females and males of the sheep species on their fertility

Using the technique of massive sequencing, interesting results over the influence of the microbiota of the reproductive tracts of females and males of the sheep species on their fertility.

Artificial insemination (AI) is an essential tool in genetic improvement programs, especially in dairy ruminants. In sheep, AI fertility has low percentages ranging between 30% and 60%, there are many factors that contribute to this fact.

The majority of fertility-related characters show very low heritability, between 1 and 4% (Andersen-Ranberg et al. 20015; Chang et al. 2006; González-Recio et al. 2006), which, together with The difficulty and time required for the collection of phenotypes, and the complexity and impression of the statistical models for their evaluation, make the selection of these characters using traditional methods inefficient.

Traditionally, the complete microbiota of the tract is not cultivable, so it has been tried to find out by other methodologies.

The INIA Animal genetic improvement group have found good conclusions about the influence of the microbiota of the reproductive tracts of females and males of the sheep species on their fertility , but there is still much to work on to clarify these issues and improve sheep production as in other species.

Information: Publication for veterinarians of Production animals, albéitar (November 2019, vol. 230)

Related posts: https://proiser.com/determine-the-semen-quality-of-three-species/

Influence of the microbiota

How do you do stain to evaluate sperm morphology?

For the correct visualization of a sperm sample it is important to know how to stain to evaluate sperm morphology. Approximately 15% of couples have infertility problems. Morphology is an important parameter for successful fertilization.

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Sperm analysis CASA system: ISAS V1

A sperm analysis CASA system is one whose fundamental objective is to perform an accurate analysis, based on the repetition and performed automatically, of some parameters that appear in a sperm sample.

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Determine the semen quality of three species of interest to science with ISAS® V1

European scientists create a system for determining the quality of individualized semen. The study, the quality of sperm samples from three species of great interest was determined for conservation reasons or for the interest of the industrial aquaculture sector.

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Combined effect of counting chamber and diluent on sperm kinematics

The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination.

The evaluation of sperm The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination. Here, the combined effect of the type and capture area of three counting chambers, together with the type of diluent employed, on sperm motility was analysed. Ejaculates from thirteen Holstein bulls were used for sperm kinematic analysis with the ISAS®v1 CASA‐Mot system, using two capillary‐loaded counting chambers (Leja® and Cell‐Vu®) and one drop displacement chamber (Makler®).

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ISASP – The most successful solution for evaluation of seminal samples

A system for evaluation of seminal samples that will help you save a lot of time in the analysis of your samples for the doses that you need.

Proiser provides you with a series of solutions for the evaluation of seminal samples of any kind. As we have previously showed, our ISASv1 software is multi-species and gives you the possibility to acquire a lot of information about the sample.

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Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence

Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence (12-18 years) and the effect of varicocele.

Volunteers between 150 and 224 months of age, who had reached oigarche by 12 years old, were recruited in the area of Barranquilla, Colombia.

Genetic, environmental, and nutritional factors have a great influence on the time to reach puberty. Therefore, it is difficult to predict the age at which a particular individual will achieve his reproductive maturity, i.e., the sperm production corresponding to that of an adult.

In addition, biomedical information about the age of first conscious ejaculation (oigarche1), the presence or absence of spermatozoa in seminal fluid, the biological characteristics of spermatozoa during this maturational process, and the time required to reach adult spermatogenesis, is currently scarce and controversial.

The few studies reported on this topic correspond to adolescents under the age of 18 years and are related to different pathologies such as testicular cancer.

The main objective of this study was to investigate sperm features in adolescents between 12 and 18 years of age. These data are part of a social project carried out in Barranquilla, Colombia, directed to develop a sexual educational program because of the high teenage pregnancy rate in this area. Therefore, it is necessary to know the mean age at the onset of sperm production in adolescents from this area to provide the best counseling.

In addition, we analyzed the effect of varicocele and used morphometric data analysis to assess sperm subpopulations in human semen samples from these adolescents.

Complete article: https://proiser.com/wp-content/uploads/2019/06/Articulo-adolescentes.pdf

Disposable chambers for cell counting . An option for your counting.

Disposable chambers for cell counting . An option for your counting.

A simple option to help in the laboratory when performing the analyzes. There are a diversity of designs and models that conform toth especifications of our customers.

Choose between D2C and D4C models, the difference is between the number of samples per camera. In both models you have to choose different depths:

  • 10 µm: The “clasic” for human semen analysis (WHO 1999).
  • 16 µm: Used in many farm seminal analysis.
  • 20 µm: Currently recommended for human semen análisis by WHO 2010. Optimal for veterinary.

Disposable chambers are indicated for concentration estimate and analysis of motility with CASA system. Pre-drawn squares assure an optimal sampling.

Disposable chambers

One box have 25 slides for 50 or 100 analysis, all depend the model for disposable chambers.

 

 

 

How sexual competition can affect the function and quality of sperm

The presence of a rival male influences the quality of sperm, according to research led by the University of Alicante (UA) and the University of Upsala (Sweden).

The presence of a rival male influences the quality of sperm, according to research led by the University of Alicante (UA) and the University of Upsala (Sweden). This work was done with adult zebra fish (Daniorerio), reveals that the spermatozoa of males exposed to high competition (two males and one female) present changes, resulting in faster and more competitive sperm. Anyway, this is not a good way of acting, since it leads to a change in the next generation with respect to DNA integrity.

The methodology followed in the investigation consisted of placing two males for two weeks with one female and two females with one male, this would give a treatment of high competitive ness and low one. The difference in both treatments was observed and a possible explanation that brings negative effects on DNA integrity is that due to the competitive ness generated in the environment there is a high production of reactive oxygen species caused by stress.

That is to say, the quality of the spermatozoa of a seminal sample does not depend on the quality of them individually, but rather on the joint action of diverse sperm subpopulations and also depends on the joint action of the individuals of the species and their sexual-competitive relationships.

The work was published in the scientific journal Journal of Evolutionary Biology.

For more information : https://noticiasdelaciencia.com/art/32419/como-la-competencia-sexual-puede-afectar-a-la-funcion-y-calidad-del-esperma

evaluate sperm morphology

Evaluate sperm morphology

How do you do stain to evaluate sperm morphology?

Approximately 15% of couples have infertility problems. Evaluate sperm morphology is an important parameter forsuccessful fertilization. In assisted reproduction techniques (Kuster et al., 2004) the Kruger criteria(1986) and the WHO classification are the most important. According to the Kruger criteria, the head,neck and tail of the sperm must be normal and 70% of the head must be covered by the acrosome.

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