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PROISER Fertility studies

Proiser develops fertility studies in freshwater fish

Proiser together with the University of Valencia develop fertility studies in sperm samples from humans and animals, and have obtained results in freshwater fish that could be useful to protect endangered species such as salt salmon.

Proiser is the name of a Valencian company whose work is carried out in the research and development of automated systems of diagnosis and automatic semen analysis.

Its origin is located in 2004, when Dr. Carles Soler (Professor of the University of Valencia in the field of Cell Biology, Functional Biology and Physical Anthropology), and computer engineer Francisco Blasco (Computer Engineer) join to develop semen analysis systems.

Proiser‘s main product line is the ISAS (Integrated Semen Analysis System) family, composed of CASA (Compuert Assisted Semen Analysis) systems, reusable and disposable cell counting chambers, and all the tools needed to perform numerous routine andrology lab analyses. Hence this company is at the forefront of what is going on in this field of biology and the environment.
The ISAS software used by Proiser is, today, the most advanced and cutting-edge solution to perform the automatic analysis of all types of semen. In fact, it is capable of analyzing more than 2000 cells per field, and more than 80,000 sperm by analysis. A job that will allow you to evaluate sperm mobility, sperm concentration, sperm morphology and other similar characteristics at the same time.

Fertility studies. Proiser products.

Currently, Proiser has a wide range of products that allow the correct manipulation, study and analysis of semen of different animals (including humans).

In addition, it develops its own lines of research such as the one that focuses its attention on the fertility of freshwater fish.

In this context, professionals from Proiser and the University of Valencia have already published results around sperm quality studies in humans and animals; as well as the results that have been obtained in freshwater fish, made with their own technique.

Particularly relevant have been its conclusions as far as salt salmon are concerned, a very important species at a commercial level and which has come to conclude, for example, that genetic-environmental interactions can be an important factor in the reproductive competition of male individuals of this species.
To carry out this work, Proiser has used a technique of his own with which they have been able to study sperm kinetics, thus identifying the different sub-populations of sperm that exist.
This work is part of Horizon 2020’s IMPRESS-Marie Sklodowska-Curie Program. A European programme that has allowed, for example, the experimental part to have been developed at the National Conservatory of Salmon Salar in the French town of Chanteuges.
PROISER ISAS Fertility Studies

 

This line of research highlights the important contribution of the Integrated Semen Analysis System (ISAS) in the study of different parameters of semen samples such as concentration, motility, percentage of progressives or different kinetic parameters and their use for scientific advancement in areas such as clinics, humans and veterinarians, scientific research, toxicology or livestock production among others.

ISAS V1

Multispecies sperm analysis: ISAS V1

ISAS V1 for Sperm tests, which are increasingly widespread evidence among the animal and human population. Hence, there is an increasing supply of systems on the market and, by extension, it is increasingly necessary to have systems that offer greater efficiency in the results obtained.
In Proiser we are aware of this, that is why we have developed the ISAS V1 method, a system that gathers tools to be used both in clinics and in research.

Its fields of application are both the field of human sciences and those related to veterinary, scientific research, livestock production or pharmaceutical and reproductive toxicology.

ISAS V1 is currently considered to be the most complete and easy-to-use sperm analysis system of all we can find on the market. This is mainly because it works under different hardware conditions and with different operating systems, so it adapts to the needs of all types of users.

Caption from DNA Fragmentation-. ISAS V1 

ISAS V1: What it is and what applications it has

ISAS V1 is a sperm analysis system suitable for multiple species, so it can work with different sperm samples. It doesn’t matter if it is to analyze the sperm sample of a man, a horse, a bull, a pig, a goat, rabbit, dog, rat, lamb, etc. It’s valid for all of them. This does not imply that ISAS V1 always applies the same parameters. On the contrary, each species has its own configuration algorithms and results in independent analysis.

Depending on the sample, ISAS may use negative or positive phase contrast. The motility analysis of a field is performed in real time (less than one second), and can be configured to analyze motility up to 250 frames, at 200 frames per second and with concentrations greater than 1000 sperm/field.

In addition to the concentration, motility and percentage of progressives, with ISAS V1 the following kinetic parameters are also calculated: VCL, VSL, VAP, LIN, STR, WOD, ALH, BCF, DNC, MAD, among others.

It should also be noted that it works with cameras up to 200 frames per second, allowing for near-perfect analysis of sperm behavior.

In addition, it is specially designed for quality control programs, allowing the analysis of video files of many different formats (Windows AVI, Windows Media Video, MPEG-4, DV among others). It also allows to obtain images, which makes it easier to have collections of videos and images for offline analysis of mobility, concentration, morphology, vitality or DNA fragmentation.

Another application of ISAS V1 is the morphological and morphometric analysis of sperm from dyed or fresh samples (using phase contrast optics).
This work provides data on 14 morphometric parameters: head size (area, length, width and perimeter), head shape (ellipticality, elongation, roughness, and regularity), percentage of acrosome, head color level, centerpiece size (area and width), and insertion of the middle part (angle and distance).

With ISAS we can also know the data related to the percentage of fragmented sperm. To do this, it uses 20x or 40x real-time (less than 1 second) targets.

Finally, it should be noted that ISAS V1 also provides data on automatic feasibility of sperm cells. This is especially interesting because we need to determine the actual percentage of viable cells in a sample, and to accurately perform this analysis, we use fluorescence techniques with their corresponding use of tying kits.

ISAS V1

Caption From Vitality test-. ISAS V1

FEATURES of the ISAS V1 system

The current ISAS V1 equipment is completely modular, so each part of it can operate independently and together.
In addition, we have designed the system to allow most of the components that can be found in each type of laboratory, from the microscope to the computer, as long as they meet the minimum system requirements.

So, if you are looking for a system with which you can do high quality multispecies sperm analysis and be able to obtain a large number of data, do not hesitate and come to Proiser. You’ll find everything you need.