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Sperm quality and subpopulation behavior.

The quality of sperm in a semen sample does not depend, as previously thought, on the individual quality of sperm samples. On the contrary, it depends on the joint action of sperm subpopulations.

 

This conclusion, which is based on a clear research premise, has been made possible by the work of researchers from the University of Valencia and the PROISER Company.

 

Sperm quality: A job of many years

The study on sperm behavior and sperm quality in different samples has been the main objective of the Department of Cell Biology, Functional and Physical Anthropology of the University of Valencia for several decades. A line of research that has been supported by the Valencian company PROISER, dedicated to the diagnosis of semen samples and which has developed revolutionary software to reveal parameters of sperm samples and give answers on sperm morphometry.

Sperm morphology is a line of study and work that is based on the correct identification of sperm involved in the reproduction function of some internal fertilization species.

An area of work in which the members of the research team of the University of Valencia raised the need to check the starting point of many of the work that had been carried out in the laboratories. Works that claimed that the success of fertilization depended on the health of a sperm and its individual ability.

However, thanks to the trials carried out by the professionals of the University of Valencia, it was possible to verify that the way sperm behave in a reproduction process is mainly due to the joint action of them and the coordination they develop with each other.

Hence the main element of work in internal in Vitro fertilization processes is no longer a single sperm, but a sample of them.

computer assisted counting. sperm quality

Sperm analysis. – CASA System

The findings of this study revolutionized the field of assisted reproduction by offering answers and solutions around male fertility with internal fertilization.

 

Reference in the field of reproduction

With this new work, which has been echoed by numerous scientific journals, a change in assisted reproduction strategy has been defined, both in its veterinary and human applications. What has made both the University of Valencia and the company PROISER a reference for the rest of the laboratories and entities that carry out their work in this field.

This new milestone adds to other PROISER findings in the same area. This private entity has been developing computer programs and applications for more than a decade that favor successful seminal analysis. In fact, it is noted as a leading company in the development of different systems of sperm analysis that in the professional field are known as “Computer Assisted Semen Analysis“. A quantification system that has full implementation today.

PROISER. sperm quality

CASA System

In this way, PROISER continues to advance and place Valencian talent at the top of the international ranking.

sperm morphology. Proiser. Trumorph.

Thrumorph: the new technique of observing sperm morphology

Trumorph is the name of a technique that is presented as the definitive mode of work in terms of observation of sperm morphology.

A system with which you will be able to obtain reliable data with which to carry out your research on sperm samples, in the best possible way. Who your lab, using this system, will be able to be more competitive and be able to respond to the needs of users faster than is usually done.

In order to know this system, its operation and advantages, we have written this article in which we want to develop a little its concept and philosophy.

Trumorph: what is it and what advantages it offers in the observation of sperm morphology.

Trumorph is a system that allows us to analyze the morphology of sperm from a semen sample of a person or animal. So thanks to it we can know all the data we need to work with sperm samples.
To achieve this, Trumorph comprises a device and a 40x/60 PH (-) lens. So, after placing a drop between the carrier and the conventional cover, the device raises the sample temperature to 45 degrees Celsius for 5 seconds, applying a slight pressure. This allows cells to be immobilized and easier to observe in the optical microscope. Which allows you to carry out your study without any problem more quickly because thanks to this system we have prepared the sample in just a few seconds.

What saves key time for this activity, as the shorter process of preparing sperm samples usually takes about 2 hours.
Trumorph®. PROISER.sperm morphology

Trumorph®

Trumorph is an innovative technique of observing sperm morphology

You only need 20 seconds to prepare the sample!

Trath significant advantage of this system, is that the results obtained in terms of observation of the sperm morphology are comparable to those observed after using conventional stains, with the advantage that in this case it is not necessary to introduce these elements.

The Trumorph system is supported by different scientific publications, as it has been proven to be valid both in the conduct of analysis with human samples and those obtained from other animals. You can request more information at info-proiser.com

On our website you can obtain information about Trumorph as well as all the product offering that Proiser has at the disposal of its customers to give a complete solution for the analysis and counting of sperm samples; sperm counting cameras; Heated plates and plates; staining kits and reagents and systems to develop analysis on sperm morphology in living cells refers.
In any case, if you have any questions and need to clarify it, do not hesitate and contact us. We will assist you both by email and by phone.

Spermtrack. seminal counting chamber. Proiser

Reusable counting chamber of sperm samples

When we work with sperm samples we need top quality products. Systems and work supplements like those we offer in Proiser, where you will be able to acquire all kinds of tools and substances that you require for your daily work. One of the most popular is our seminal counting chamber. A product that is called Spermtrack and that allows to alleviate the workload of laboratories.

To get to know it, in Proiser we have written this article with which we want to explain its main characteristics and its operation.

Seminal counting chamber: Spermtrack

Spermtrack is the name of a new counting chamber that has launched the ISAS brand, one of the most prestigious in the scientific field.

Thank you to this new camera, seminal analysis is developed as an activity with a new tool that substantially improves pre-existing counting chambers.

And is that following the semen drop-shifting procedure, Spermtrack eliminates most of the disadvantages presented by the less efficient products found on the market.

The Spermtrack design makes it easy to use under the microscope and also the cleaning, handling and storage actions that require proper use.

Spermtrack. seminal counting chamber PROISER

Spermtrack®

 

Advantages of using Spermtrack

To facilitate its handling, Spermtrack can be customized with different colors. Allowing even recording a name at the bottom of the camera. So we avoid mistakes or have to repeat tests because samples have been mixed.

To adapt to all the needs and activities around this activity, Spermtrack is available in two models: one of 10 and one of 20 microns. In the first case, it is a 10 μm depth chamber for traditional human clinical analysis. On the second model, it should be noted that it is a 20 μm  chamber, which follows the latest recommendations from the 2010 WHO Laboratory Manual, while expanding its use for veterinary purposes, due to increased natural freedom of movement of sperm cells.

SPERMTRAK. PROISER.

Spermtrack®

Another advantage offered by the use of this camera is its traceability as far as its daily application is concerned is that it has independent series numbers on the lid and in the camera, allowing for complete traceability according to ISO 13485 standards. A guarantee for those who work with them.
It should be noted, too, that Spermtrack is thinner than other similar products. This allows the microscope capacitor light to reach the sample much more focused, improving optical quality and preventing the generation of common visual artifacts that appear on other similar devices. All of this makes it easier for people who manipulate them to get their results more reliably.
So, if you want your lab to be more competitive when it comes to sperm sample work, don’t hesitate and purchase these reusable counting chambers. You will be able to find them on our website, in the tab that we dedicate to the products that relate to the seminal analysis. Here, in addition, you will be able to get more data about it, and even see photographs of the product. Which will allow you to get a visual idea of what it’s like and how you work with it.
spermtrack seminal counting chamber. PROISER

Spermtrack®

And if despite all this you still have doubts about anything related to it, do not hesitate and contact us, Proiser. We’ll figure it out for you quickly.

PROISER Fertility studies

Proiser develops fertility studies in freshwater fish

Proiser together with the University of Valencia develop fertility studies in sperm samples from humans and animals, and have obtained results in freshwater fish that could be useful to protect endangered species such as salt salmon.

Proiser is the name of a Valencian company whose work is carried out in the research and development of automated systems of diagnosis and automatic semen analysis.

Its origin is located in 2004, when Dr. Carles Soler (Professor of the University of Valencia in the field of Cell Biology, Functional Biology and Physical Anthropology), and computer engineer Francisco Blasco (Computer Engineer) join to develop semen analysis systems.

Proiser‘s main product line is the ISAS (Integrated Semen Analysis System) family, composed of CASA (Compuert Assisted Semen Analysis) systems, reusable and disposable cell counting chambers, and all the tools needed to perform numerous routine andrology lab analyses. Hence this company is at the forefront of what is going on in this field of biology and the environment.
The ISAS software used by Proiser is, today, the most advanced and cutting-edge solution to perform the automatic analysis of all types of semen. In fact, it is capable of analyzing more than 2000 cells per field, and more than 80,000 sperm by analysis. A job that will allow you to evaluate sperm mobility, sperm concentration, sperm morphology and other similar characteristics at the same time.

Fertility studies. Proiser products.

Currently, Proiser has a wide range of products that allow the correct manipulation, study and analysis of semen of different animals (including humans).

In addition, it develops its own lines of research such as the one that focuses its attention on the fertility of freshwater fish.

In this context, professionals from Proiser and the University of Valencia have already published results around sperm quality studies in humans and animals; as well as the results that have been obtained in freshwater fish, made with their own technique.

Particularly relevant have been its conclusions as far as salt salmon are concerned, a very important species at a commercial level and which has come to conclude, for example, that genetic-environmental interactions can be an important factor in the reproductive competition of male individuals of this species.
To carry out this work, Proiser has used a technique of his own with which they have been able to study sperm kinetics, thus identifying the different sub-populations of sperm that exist.
This work is part of Horizon 2020’s IMPRESS-Marie Sklodowska-Curie Program. A European programme that has allowed, for example, the experimental part to have been developed at the National Conservatory of Salmon Salar in the French town of Chanteuges.
PROISER ISAS Fertility Studies

 

This line of research highlights the important contribution of the Integrated Semen Analysis System (ISAS) in the study of different parameters of semen samples such as concentration, motility, percentage of progressives or different kinetic parameters and their use for scientific advancement in areas such as clinics, humans and veterinarians, scientific research, toxicology or livestock production among others.