Posts

How do you do stain to evaluate sperm morphology?

For the correct visualization of a sperm sample it is important to know how to stain to evaluate sperm morphology. Approximately 15% of couples have infertility problems. Morphology is an important parameter for successful fertilization.

Read more

Combined effect of counting chamber and diluent on sperm kinematics

The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination.

The evaluation of sperm The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination. Here, the combined effect of the type and capture area of three counting chambers, together with the type of diluent employed, on sperm motility was analysed. Ejaculates from thirteen Holstein bulls were used for sperm kinematic analysis with the ISAS®v1 CASA‐Mot system, using two capillary‐loaded counting chambers (Leja® and Cell‐Vu®) and one drop displacement chamber (Makler®).

Read more

evaluate sperm morphology

Evaluate sperm morphology

How do you do stain to evaluate sperm morphology?

Approximately 15% of couples have infertility problems. Evaluate sperm morphology is an important parameter forsuccessful fertilization. In assisted reproduction techniques (Kuster et al., 2004) the Kruger criteria(1986) and the WHO classification are the most important. According to the Kruger criteria, the head,neck and tail of the sperm must be normal and 70% of the head must be covered by the acrosome.

Read more

espermatozoide

Sperm quality and behavior of subpopulations.

Sperm quality and subpopulations behaviour.

The quality of the sperm from a semen sample does not depend, as was previously thought, on the individual quality of the semen, this depend the joint action of many sperm subpopulations.

espermatozoide
This conclusion, which is based on research carried out in recent decades by various research groups, has been corroborated by the joint work of researchers from the University of Valencia and the PROISER R + D, S.L.

The study on the sperm behaviour and their quality in samples of diverse species has been one of the main objectives of the research group by Dr. Carles Soler in the Departament of Celular biology, Functional Biology and Physical Anthropology of the Universitat de València for several decades. A line of research that has been linked to the approaches of I D I from the Valencian company PROISER, dedicated to the development of solutions for the diagnosis of semen samples. They have developed a revolutionary software to study the divers quantitative parameters of sperm samples.

As a result of the experience acquired, Dr. Carles Soler, as head of the University’s research group and as scientific director of PROISER, was invited to be the editor of two special issues in the prestigious journals Asian Journal of Andrology (2016) and Reproduction, Fertility and Development (2018), respectively, with morphometry and sperm kinetics.

The CASA systems (Computer Assisted Semen Analysis) developed by PROISER provides a databases for tens of thousands of sperm. The application of advanced statistical techniques of multivariate analysis (main components and cluster) from these databases have allowed to consolidate the concept of subpopulations.

Hitherto, has been thought that the behavior of sperm within an ejaculate was comparable to what happens in a marathon: many runners, in principle equivalent, competing for the goal that is the oocyte. Now, the verification mathematically coherent subpopulations points more or less organized team concept.

All of this is around about what happen the sperm in a female tract. Each spermatozoonfind many obstacules and barriers that help to select the best sperm. We can say that it is similar to chess. It would not make sense to spend the towers or even the king in the opening and the first dabbling, that’s what the pawns are for.

espermatozoideIn any case, we still do not know what the rules that the sperm through in the female genital tract. The essay by the Universitat de València and PROISER, are currently work for to find more solutions.

 

Referent in the reproduction area

In addition to the physiological conditions, these results should lead to the definition of changes in the strategies followed in assisted reproduction, both in their veterinary and human applications. This group of studies and the new perspectives that have consider turned the group of the Universitat de València and PROISER into world leaders.

This new points come in addition to other PROISER findings in the same area. It is this private company has been developing software and computer applications for more than a decade that help to do a successful seminal analysis.

In this way, PROISER continues to advance and placing Valencian talent at the top of the international ranking.