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ISAS V1

Multispecies sperm analysis: ISAS V1

ISAS V1 for Sperm tests, which are increasingly widespread evidence among the animal and human population. Hence, there is an increasing supply of systems on the market and, by extension, it is increasingly necessary to have systems that offer greater efficiency in the results obtained.
In Proiser we are aware of this, that is why we have developed the ISAS V1 method, a system that gathers tools to be used both in clinics and in research.

Its fields of application are both the field of human sciences and those related to veterinary, scientific research, livestock production or pharmaceutical and reproductive toxicology.

ISAS V1 is currently considered to be the most complete and easy-to-use sperm analysis system of all we can find on the market. This is mainly because it works under different hardware conditions and with different operating systems, so it adapts to the needs of all types of users.

Caption from DNA Fragmentation-. ISAS V1 

ISAS V1: What it is and what applications it has

ISAS V1 is a sperm analysis system suitable for multiple species, so it can work with different sperm samples. It doesn’t matter if it is to analyze the sperm sample of a man, a horse, a bull, a pig, a goat, rabbit, dog, rat, lamb, etc. It’s valid for all of them. This does not imply that ISAS V1 always applies the same parameters. On the contrary, each species has its own configuration algorithms and results in independent analysis.

Depending on the sample, ISAS may use negative or positive phase contrast. The motility analysis of a field is performed in real time (less than one second), and can be configured to analyze motility up to 250 frames, at 200 frames per second and with concentrations greater than 1000 sperm/field.

In addition to the concentration, motility and percentage of progressives, with ISAS V1 the following kinetic parameters are also calculated: VCL, VSL, VAP, LIN, STR, WOD, ALH, BCF, DNC, MAD, among others.

It should also be noted that it works with cameras up to 200 frames per second, allowing for near-perfect analysis of sperm behavior.

In addition, it is specially designed for quality control programs, allowing the analysis of video files of many different formats (Windows AVI, Windows Media Video, MPEG-4, DV among others). It also allows to obtain images, which makes it easier to have collections of videos and images for offline analysis of mobility, concentration, morphology, vitality or DNA fragmentation.

Another application of ISAS V1 is the morphological and morphometric analysis of sperm from dyed or fresh samples (using phase contrast optics).
This work provides data on 14 morphometric parameters: head size (area, length, width and perimeter), head shape (ellipticality, elongation, roughness, and regularity), percentage of acrosome, head color level, centerpiece size (area and width), and insertion of the middle part (angle and distance).

With ISAS we can also know the data related to the percentage of fragmented sperm. To do this, it uses 20x or 40x real-time (less than 1 second) targets.

Finally, it should be noted that ISAS V1 also provides data on automatic feasibility of sperm cells. This is especially interesting because we need to determine the actual percentage of viable cells in a sample, and to accurately perform this analysis, we use fluorescence techniques with their corresponding use of tying kits.

ISAS V1

Caption From Vitality test-. ISAS V1

FEATURES of the ISAS V1 system

The current ISAS V1 equipment is completely modular, so each part of it can operate independently and together.
In addition, we have designed the system to allow most of the components that can be found in each type of laboratory, from the microscope to the computer, as long as they meet the minimum system requirements.

So, if you are looking for a system with which you can do high quality multispecies sperm analysis and be able to obtain a large number of data, do not hesitate and come to Proiser. You’ll find everything you need.

 

Combined effect of counting chamber and diluent on sperm kinematics

The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination.

The evaluation of sperm The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination. Here, the combined effect of the type and capture area of three counting chambers, together with the type of diluent employed, on sperm motility was analysed. Ejaculates from thirteen Holstein bulls were used for sperm kinematic analysis with the ISAS®v1 CASA‐Mot system, using two capillary‐loaded counting chambers (Leja® and Cell‐Vu®) and one drop displacement chamber (Makler®).

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evaluate sperm morphology

Evaluate sperm morphology

How do you do stain to evaluate sperm morphology?

Approximately 15% of couples have infertility problems. Evaluate sperm morphology is an important parameter forsuccessful fertilization. In assisted reproduction techniques (Kuster et al., 2004) the Kruger criteria(1986) and the WHO classification are the most important. According to the Kruger criteria, the head,neck and tail of the sperm must be normal and 70% of the head must be covered by the acrosome.

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espermatozoide

Sperm quality and behavior of subpopulations.

Sperm quality and subpopulations behaviour.

The quality of the sperm from a semen sample does not depend, as was previously thought, on the individual quality of the semen, this depend the joint action of many sperm subpopulations.

espermatozoide
This conclusion, which is based on research carried out in recent decades by various research groups, has been corroborated by the joint work of researchers from the University of Valencia and the PROISER R + D, S.L.

The study on the sperm behaviour and their quality in samples of diverse species has been one of the main objectives of the research group by Dr. Carles Soler in the Departament of Celular biology, Functional Biology and Physical Anthropology of the Universitat de València for several decades. A line of research that has been linked to the approaches of I D I from the Valencian company PROISER, dedicated to the development of solutions for the diagnosis of semen samples. They have developed a revolutionary software to study the divers quantitative parameters of sperm samples.

As a result of the experience acquired, Dr. Carles Soler, as head of the University’s research group and as scientific director of PROISER, was invited to be the editor of two special issues in the prestigious journals Asian Journal of Andrology (2016) and Reproduction, Fertility and Development (2018), respectively, with morphometry and sperm kinetics.

The CASA systems (Computer Assisted Semen Analysis) developed by PROISER provides a databases for tens of thousands of sperm. The application of advanced statistical techniques of multivariate analysis (main components and cluster) from these databases have allowed to consolidate the concept of subpopulations.

Hitherto, has been thought that the behavior of sperm within an ejaculate was comparable to what happens in a marathon: many runners, in principle equivalent, competing for the goal that is the oocyte. Now, the verification mathematically coherent subpopulations points more or less organized team concept.

All of this is around about what happen the sperm in a female tract. Each spermatozoonfind many obstacules and barriers that help to select the best sperm. We can say that it is similar to chess. It would not make sense to spend the towers or even the king in the opening and the first dabbling, that’s what the pawns are for.

espermatozoideIn any case, we still do not know what the rules that the sperm through in the female genital tract. The essay by the Universitat de València and PROISER, are currently work for to find more solutions.

 

Referent in the reproduction area

In addition to the physiological conditions, these results should lead to the definition of changes in the strategies followed in assisted reproduction, both in their veterinary and human applications. This group of studies and the new perspectives that have consider turned the group of the Universitat de València and PROISER into world leaders.

This new points come in addition to other PROISER findings in the same area. It is this private company has been developing software and computer applications for more than a decade that help to do a successful seminal analysis.

In this way, PROISER continues to advance and placing Valencian talent at the top of the international ranking.